Crystallographic Studies of Chemically Modified Nucleic Acids: A Backward Glance

Potentiometric sensing of nucleic acids using chemically modified nanopores.

Unlike the overwhelming majority of nanopore sensors that are based on the measurement of a transpore ionic current, here we introduce a potentiometric sensing scheme and demonstrate its application for the selective detection of nucleic acids. The sensing concept uses the charge inversion that occurs in the sensing zone of a nanopore upon binding of negatively charged microRNA strands to posit...

Chemically modified nucleic acids as immunodetectable probes in hybridization experiments.

Guanine residues in nucleic acids can be modified by treatment with N-acetoxy-N-2-acetylaminofluorene and its 7-iodo derivative in an in vitro nonenzymatic reaction. The modified nucleic acids (ribo or deoxyribo, single or double stranded) are recognized by specific antibodies. They can be immunoprecipitated or used as probes in hybridization experiments and detected by immunochemical techniques.

Chromatographic Studies on Nucleic Acids

The nucleic acids are such universal constituents of biological systems and their function is probably so important that it is not surprising that much labour has been spent on the study of their composition. Until very recently there have been no methods of analysis which could be considered more than semiquantitative, and it now seems doubtful whether many published analyses are even approxim...

Crystallographic model quality at a glance

A crystallographic macromolecular model is typically characterized by a list of quality criteria, such as R factors, deviations from ideal stereochemistry and average B factors, which are usually provided as tables in publications or in structural databases. In order to facilitate a quick model-quality evaluation, a graphical representation is proposed. Each key parameter such as R factor or bo...

Structural and Biophysical Studies of Nucleic Acids